Ku protein
Abstract. Human Ku70 is a well-known endogenous nuclear protein involved in the non-homologous end joining pathway to repair double-stranded breaks in DNA. However, Ku70 has been studied in multiple contexts and grown into a multifunctional protein. In addition to the extensive functional study of Ku70 in DNA repair process, many studies have ...Introduction. Ku protein is an important component of the nonhomologous end-joining (NHEJ) pathway of DNA double-strand break repair, and it is ubiquitous among eukaryotes. 1-5 Some bacterial species encode a homolog of Ku, and bacterial Ku proteins function along with a dedicated ligase to repair DNA double-strand breaks and protect against genome rearrangements (for review, see Ref. 6).AltEJ is independent of the DNA binding Ku protein complex and requires DNA end resection. Pol θ is able to mediate joining of two resected 3' ends harboring DNA sequence microhomology. "Signatures" of Pol θ action during altEJ are the frequent utilization of longer microhomologies, and the insertion of additional sequences at joining sites.
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The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK.Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins.S1). In contrast to eukaryotes, mycobacteria such as Mycobacteria marinum possess only a single small Ku protein of 291 amino acids which forms a Ku homodimer for binding DNA ends and NHEJ repair ...Jun 18, 2023 · Nature子刊：中山大学松阳洲团队发现DNA损伤修复通路的新选择. DNA双链断裂 （DNA double-strand break，DSB）是一种常见且高度有害的DNA损伤形式，严重威胁着基因组稳定性和细胞存活。. DSB修复缺陷或失调与肿瘤、衰老以及免疫缺陷等密切相关。. 非同源末端连接（Non ...© STRING Consortium 2023. SIB - Swiss Institute of Bioinformatics; CPR - Novo Nordisk Foundation Center Protein Research; EMBL - European Molecular Biology Laboratory
Ku is an abundant, highly conserved DNA binding protein found in both prokaryotes and eukaryotes that plays essential roles in the maintenance of genome integrity. In eukaryotes, Ku is a heterodimer comprised of two subunits, Ku70 and Ku80, that is best characterized for its central role as the initial DNA end binding factor in the "classical" non-homologous end joining (C-NHEJ) pathway ...However, in some circumstances DSBs are repaired by a Ku-independent repair mechanism that use microhomology as an intermediate and is dependent on ligase 3 ... Dynan WS, Yoo S (1998) Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids. Nucleic Acids Res 26: 1551–1559. View ArticleAravind L, Koonin EV . 2001 Prokaryotic homologs of the eukaryotic DNA-end-binding protein Ku, novel domains in the Ku protein and prediction of a prokaryotic double-strand break repair system ...Ku protein, a heterodimer of 70 kDa and 83 kDa polypeptides, is the regulatory component of the DNA-dependent protein kinase (DNA-PK). Ku protein binds to DNA discontinuities and is essential for DNA double-strand break repair.These results suggest that Ku proteins are specific to breast cancer progression and indicate that in intestinal cancer cells, tumoregenesis is not associated with Ku protein expression. Many studies have suggested a key role of Ku in cancer development and progression, acting as either tumor suppressor gene or as oncogene 45 .
The increase in the levels of Ku protein was comparable to the increase in the binding activity seen in the EMSA experiments. Thus, there is an inverse relationship between αMyHC expression (mRNA) and Ku protein in myocardial pathological hypertrophy and failure. Interestingly, Ku80 protein levels were not increased in human failing hearts ...The DNA-dependent protein kinase (DNA-PK) is a serine/threonine protein kinase composed of a large catalytic subunit (DNA-PKcs) and the Ku70/80 heterodimer. Over the past two decades, significant progress has been made in elucidating the role of DNA-PK in non-homologous end joining (NHEJ), the major pathway for repair of ionizing radiation-induced DNA double strand breaks in human cells and ...Unlike E. coli, Mycobacterium tuberculosis (Mt) expresses a Ku-like protein and an ATP-dependent DNA ligase that can perform non-homologous end-joining (NHEJ). We have expressed the Mt-Ku and Mt-Ligase D in E. coli using an arabinose-inducible promoter and expression vectors that integrate into specific sites in the E. coli chromosome.E. coli strains have been generated that express the Mt-Ku ... ….
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Ku is a dimeric protein complex that binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. Ku is evolutionarily conserved from bacteria to humans. The ancestral bacterial Ku is a homodimer (two copies of the same protein bound to each other). [2]Homotypic and heterotypic protein associations control Rad51 function in double-strand break repair. Genes Dev. 1994 Nov 1; 8 (21):2552-2562. [Google Scholar] Dvir A, Peterson SR, Knuth MW, Lu H, Dynan WS. Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II.The Ku protein-DNA-dependent protein kinase system is one of the major pathways by which cells of higher eukaryotes respond to double-strand DNA breaks. The components of the system are evolutionarily conserved and homologs are known from a number of organisms. The Ku protein component binds directly to DNA ends and may help align them for ...
The F-box protein Fbxl12 binds to DSBs in a Ku-sensitive manner. (A) 35 S-labeled Xenopus tropicalis Fbxl12 and histone H3 were added to egg extract that was either mock- or Ku-depleted. Streptavidin-coated (SA), SB-DNA, or DB-DNA beads were added to the mock-depleted extract and SB-DNA beads were added to the Ku-depleted extract.Although Ku protein stabilizes DNA-PKcs binding overall, it is not required for discrimination between terminal structures. Base mispairing, temperature and the presence of an interstrand linkage influence the stability of the initial complex in a manner that suggests a requirement for DNA unwinding, reminiscent of the 'open complex' model of ...
mark and abbie taylor kansas city Jul 1, 2006 · The web server frees users from complex installation of database-dependent parallel software and maintaining large hardware resources needed for protein docking simulations. Docking problems submitted to GRAMM-X server are processed by a 320 processor Linux cluster. The server was extensively tested by benchmarking, several …Jun 15, 2017 · Subsequent work established that effective DNA-PKcs recruitment and activation by DNA require Ku, a heterodimer comprising two subunits of ∼70 and 80 kDa (Dvir et al., 1992, ... One such protein is the p53 co-factor hnRNP K, which is phosphorylated by ATM in response to DSBs and protected from MDM2-mediated degradation (Moumen … how to advocateshanna khan instagram multifunctional ATP-dependent Ligase-D and Ku protein that collectively protect, process, and ligate DNA ends (13,14). Other bacteria, such as70 the prevalent gut . 71. commensal . Escherichia coli. where Ligase-D-like and Ku-like proteins have not been . 72. found are generally accepted to be NHEJ-free (12) and rely only on HR to repair . 73 ... kansas draft picks 2023 The observed effects cannot be accounted for by the differential expression levels of Ku protein in control and SC4 aptamer-expressing cells, but are rather due to augmented DNA binding-capacity of Ku protein, as demonstrated in in vitro studies. Thus, RNA aptamers against Ku protein show potential to sensitize MCF-7 breast carcinoma cells to ...The Ku protein, a DNA-binding complex that is composed of two subunits of 70 kDa and of 86 kDa, has been suggested to play a role in gene transcription. The dependence of the in vitro DNA-binding activity of affinity-purified Ku protein on reduced cysteine residues has been studied using sulphydryl-modifying agents. 5 community resourcesmike edgarlu parking pass The Ku complex, a heterodimer of 86- and 70-kD proteins, is a nuclear DNA-binding autoantigen. However, hydrophobicity analysis of the deduced amino acid sequence of the 70-kD protein had strongly suggested that this might also be a membrane protein.Here, we report that the Ku proteins (Ku80 and Ku70) directly interact with cGAS and positively regulate cGAS-mediated antiviral signaling. Mechanistically, we find that the interaction of the Ku proteins with cGAS significantly increases the DNA-binding affinity of cGAS and promotes cGAS condensation in the cytosol, thereby enhancing cGAS ... craigslist royal palm beach Besides these inhibiting molecules, HDR can also be promoted using small interfering RNA (siRNA) to inhibit the expression of Ku protein, which is the pioneer protein in the NHEJ pathway. Li et al. assessed this method on pig fetal fibroblasts (Li et al., 2018). The result showed that by inhibiting Ku70 or Ku80 expression, HR can be promoted by ...In addition to directly binding telomeric DNA, human Ku also interacts with protein components of the shelterin complex - TRF1, TRF2 and Rap1 53,54,55,56,57. Thus, Ku's DEB activity may also be ... what city is the university of kansas inpatrick joynermissouri star quilt company forum 1. Introduction. The problem of predicting folding rates (kf) for proteins with two-state and multi-state kinetics is still important and extensively studied [1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20].Many articles are devoted to the study of protein folding rates and their correlation with various structural parameters [2,6,7,8,16].In 1998, a relative contact order (rCO) parameter ...The dissection of the Ku protein from eukaryotes and prokaryotes into individual domains described above suggests an evolutionary scenario for these proteins. The Ku core is an ancient domain that was probably present in bacteria and archea even before the advent of the eukaryotes. There are clear indications that, in these organisms, the Ku ...